| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baldwin, Natalie Q (8)
|
VASRVA |
37.92 |
37.65 |
-0.27 |
| 2 |
Brock, Lydia E (8)
|
VASRVA |
38.21 |
38.45 |
0.24 |
| 3 |
Shoulders, Carrington E (8)
|
VAQSTS |
39.50 |
40.66 |
1.16 |
| 4 |
Lyons, Katharine K (8)
|
VASRVA |
43.07 |
43.32 |
0.25 |
| 5 |
Basmajian, Kensington A (8)
|
VASRVA |
43.45 |
44.43 |
0.98 |
| 6 |
Wagner, Madelyn G (7)
|
VASRVA |
46.93 |
44.89 |
-2.04 |
| 7 |
Djordjevic, Natalija (8)
|
VASRVA |
1:01.89 |
45.12 |
-16.77 |
| 8 |
Tobin, Elise M (8)
|
NCEAC |
44.64 |
47.69 |
3.05 |
| 9 |
Smith, Sloane A (7)
|
VAQSTS |
|
47.78 |
- |
| 10 |
Ednie, Elizabeth E (7)
|
VASRVA |
51.17 |
48.08 |
-3.09 |
| 11 |
Atkinson, Vivianne H (8)
|
VASRVA |
47.30 |
52.33 |
5.03 |
| 11 |
Halligan, Molly Q (8)
|
VASRVA |
55.40 |
52.33 |
-3.07 |
|
Pozzi, Anais L (8)
|
VASRVA |
48.36 |
NS |
- |